Graduate Courses BIOE 2384

BIOE 2384 - NMR Spectroscopy in medicine

Instructor: Pravat K Mandal, website,

Credits: 3

Term: fall

Description:

This course consists of two parts.

First part: Intended to teach basis understanding of NMR spectroscopy with numerous examples. Focus will be given to design different NMR experiments for drug discovery, protein structure determination and dynamics of protein molecule. Special emphasis will be given on product operator formalism to explain two dimensional and three dimensional NMR experiments.
Second part: Brain imaging using magnetic resonance spectroscopy (MRS) methodology and their application to different neurodegenerative diseases such as Alzheimer and Parkinson disease will be discussed.

Prerequisites: Students with previous simple NMR experiments will be an added advantage in this course. Mathematical background (to some extent) is a prerequisite. Strong enthusiasm and interest for learning NMR methodology is an important requirement for this course.

Required Texts: Handouts

References (on reserve in the Engineering Library):

• Pravat K. Mandal, and Majumdar A, "Comprehensive Discussion of HMQC and HSQC pulse Sequences" Concepts in Magnetic Resonance,20A, 1-24 (2004)
• Pravat K. Mandal, Pettegrew JW "Alzheimer's disease: soluble oligomeric A?(1.40) peptide in membrane mimic environment from solution NMR and circular dichroism studies" Neurochemical Research, 29, 2267-2272, 2004
• Protein NMR Spectroscopy, Cavanagh J, et al, Academic Press 1996.
• Van de Ven F. 1995. Multidimensional NMR in liquids. Basic principles and experimental methods. New York: VCH.

Course Objectives:

• NMR methodology development in a systematic fashion.
• It is expected that this course will help students to design their own NMR experiments in future.

Class/Labratory Schedule:

Monday and Friday, from 2:30-3:45 P.M